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addgene 44361 aavrg  (Addgene inc)


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    Addgene inc addgene 44361 aavrg
    Addgene 44361 Aavrg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 741 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+44361+aavrg/pAAV-hSyn-DIO-hM3D(Gq)-mCherry+(Plasmid+%2344361)/pmc13045551-44-51-51
    Average 96 stars, based on 741 article reviews
    addgene 44361 aavrg - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: Norepinephrine neurons in the locus coeruleus and the nucleus of the solitary tract drive different stress-related behavioral outputs in mice
    Article Snippet: .. To induce the expression of designer receptors exclusively activated by designer drugs (DREADDs) in PVN-projecting NE neurons, DBH-CRE mice underwent stereotaxic surgery for the bilateral intra-PVN administration of a retrograde adeno-associated virus (AAV rg ) expressing a Cre-dependent, Gq-coupled DREADD (AAV rg -hSyn-DIO-hM3D(G q )-mCherry , 2.1 × 10 13 GC/mL, Addgene 44361-AAVrg). .. For DREADD induction in NE neurons, DBH-CRE mice were subjected to stereotaxic injection of a Cre-dependent, Gq-DREADD-expressing virus, AAV9-hSyn-DIO-hM3D(G q )-mCherry (0.66 × 6 13 GC/mL, Addgene 44361-AAV9), or Gi-DREADD-expressing virus AAV9-hSyn-DIO-hM4D(Gi)-mCherry (0.33 ∗ 9 13 GC/mL, Addgene 50475-AAV9) ( ) in the bilateral LC or NTS.

    Virus:

    Article Title: Norepinephrine neurons in the locus coeruleus and the nucleus of the solitary tract drive different stress-related behavioral outputs in mice
    Article Snippet: .. To induce the expression of designer receptors exclusively activated by designer drugs (DREADDs) in PVN-projecting NE neurons, DBH-CRE mice underwent stereotaxic surgery for the bilateral intra-PVN administration of a retrograde adeno-associated virus (AAV rg ) expressing a Cre-dependent, Gq-coupled DREADD (AAV rg -hSyn-DIO-hM3D(G q )-mCherry , 2.1 × 10 13 GC/mL, Addgene 44361-AAVrg). .. For DREADD induction in NE neurons, DBH-CRE mice were subjected to stereotaxic injection of a Cre-dependent, Gq-DREADD-expressing virus, AAV9-hSyn-DIO-hM3D(G q )-mCherry (0.66 × 6 13 GC/mL, Addgene 44361-AAV9), or Gi-DREADD-expressing virus AAV9-hSyn-DIO-hM4D(Gi)-mCherry (0.33 ∗ 9 13 GC/mL, Addgene 50475-AAV9) ( ) in the bilateral LC or NTS.

    Bioprocessing:

    Article Title: Norepinephrine neurons in the locus coeruleus and the nucleus of the solitary tract drive different stress-related behavioral outputs in mice
    Article Snippet: .. To induce the expression of designer receptors exclusively activated by designer drugs (DREADDs) in PVN-projecting NE neurons, DBH-CRE mice underwent stereotaxic surgery for the bilateral intra-PVN administration of a retrograde adeno-associated virus (AAV rg ) expressing a Cre-dependent, Gq-coupled DREADD (AAV rg -hSyn-DIO-hM3D(G q )-mCherry , 2.1 × 10 13 GC/mL, Addgene 44361-AAVrg). .. For DREADD induction in NE neurons, DBH-CRE mice were subjected to stereotaxic injection of a Cre-dependent, Gq-DREADD-expressing virus, AAV9-hSyn-DIO-hM3D(G q )-mCherry (0.66 × 6 13 GC/mL, Addgene 44361-AAV9), or Gi-DREADD-expressing virus AAV9-hSyn-DIO-hM4D(Gi)-mCherry (0.33 ∗ 9 13 GC/mL, Addgene 50475-AAV9) ( ) in the bilateral LC or NTS.



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    addgene 44361 aavrg  (Addgene inc)
    96
    Addgene inc addgene 44361 aavrg
    Addgene 44361 Aavrg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+44361+aavrg/pAAV-hSyn-DIO-hM3D(Gq)-mCherry+(Plasmid+%2344361)/pmc13045551-44-51-51
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    retrograde aav virus addgene id 44361-aavrg  (Addgene inc)
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    Addgene inc retrograde aav virus addgene id 44361-aavrg
    a, b Optogenetic activation of Piezo2-expressing thoracolumbar dorsal root ganglia (DRG) neurons increases colonic mechanical sensitivity in male mice but not in female mice. n = 3 biologically independent animals for each group. Data are presented as mean values + /- SEM. Two-tailed paired t test (male: p = 0.0084, t = 10.83, r = 0.9973; female: p = 0.0751, t = 3.441, r = 0.9589). c, d <t>Retrograde</t> adeno-associated viruses <t>(AAV)</t> carrying floxed stop hM3Dq is injected into the muscular wall of the distal colon of Piezo2-Cre mice to specially label colonic afferent neurons. Clozapine N-oxide (CNO, 2 mg/kg body weight intraperitoneal (i.p.) injection) treatment causes colonic hypersensitivity in male mice ( n = 4 biologically independent animals. Data are presented as mean values + /- SEM. Two-tailed paired t test: p = 0.002, t = 7.171, r = 0.7982) but not in female mice ( n = 3 biologically independent animals. Data are presented as mean values + /- SEM. Two-tailed paired t test: p = 0.2426 , t = 1.64, r = 0.9998, no multiple comparisons were made). e Representative images of Piezo2 expression demonstrated by Piezo2-Cre driven mCherry products in DRGs of male and female mice. Scale bar: 50 μm. f Piezo2-immunoreactivity (IR) in DRG of male and female mice. Scale bar: 50 μm. g Summarized data shows the number of Piezo2-expressing neurons in 10 5 µm 2 area of DRG sections of male and female mice. Data are pools from Piezo2;mCherry (male n = 3 biologically independent animals, female n = 2 biologically independent animals) and Piezo2-IR (male n = 2 biologically independent animals, female n = 2 biologically independent animals) totaling n = 5 biologically independent male mice and n = 4 biologically independent female mice. Data are presented as mean values + /- SEM. Two-tailed unpaired t test ( p = 0.0021, t = 4.733, F = 12.83).
    Retrograde Aav Virus Addgene Id 44361 Aavrg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+44361+aavrg/aav+retrograde+virus+encoding+gfp+directed+cag+promoter/pmc10105732-311-1-4
    Average 90 stars, based on 1 article reviews
    retrograde aav virus addgene id 44361-aavrg - by Bioz Stars, 2026-11
    90/100 stars
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    a, b Optogenetic activation of Piezo2-expressing thoracolumbar dorsal root ganglia (DRG) neurons increases colonic mechanical sensitivity in male mice but not in female mice. n = 3 biologically independent animals for each group. Data are presented as mean values + /- SEM. Two-tailed paired t test (male: p = 0.0084, t = 10.83, r = 0.9973; female: p = 0.0751, t = 3.441, r = 0.9589). c, d Retrograde adeno-associated viruses (AAV) carrying floxed stop hM3Dq is injected into the muscular wall of the distal colon of Piezo2-Cre mice to specially label colonic afferent neurons. Clozapine N-oxide (CNO, 2 mg/kg body weight intraperitoneal (i.p.) injection) treatment causes colonic hypersensitivity in male mice ( n = 4 biologically independent animals. Data are presented as mean values + /- SEM. Two-tailed paired t test: p = 0.002, t = 7.171, r = 0.7982) but not in female mice ( n = 3 biologically independent animals. Data are presented as mean values + /- SEM. Two-tailed paired t test: p = 0.2426 , t = 1.64, r = 0.9998, no multiple comparisons were made). e Representative images of Piezo2 expression demonstrated by Piezo2-Cre driven mCherry products in DRGs of male and female mice. Scale bar: 50 μm. f Piezo2-immunoreactivity (IR) in DRG of male and female mice. Scale bar: 50 μm. g Summarized data shows the number of Piezo2-expressing neurons in 10 5 µm 2 area of DRG sections of male and female mice. Data are pools from Piezo2;mCherry (male n = 3 biologically independent animals, female n = 2 biologically independent animals) and Piezo2-IR (male n = 2 biologically independent animals, female n = 2 biologically independent animals) totaling n = 5 biologically independent male mice and n = 4 biologically independent female mice. Data are presented as mean values + /- SEM. Two-tailed unpaired t test ( p = 0.0021, t = 4.733, F = 12.83).

    Journal: Nature Communications

    Article Title: Piezo2 regulates colonic mechanical sensitivity in a sex specific manner in mice

    doi: 10.1038/s41467-023-37683-7

    Figure Lengend Snippet: a, b Optogenetic activation of Piezo2-expressing thoracolumbar dorsal root ganglia (DRG) neurons increases colonic mechanical sensitivity in male mice but not in female mice. n = 3 biologically independent animals for each group. Data are presented as mean values + /- SEM. Two-tailed paired t test (male: p = 0.0084, t = 10.83, r = 0.9973; female: p = 0.0751, t = 3.441, r = 0.9589). c, d Retrograde adeno-associated viruses (AAV) carrying floxed stop hM3Dq is injected into the muscular wall of the distal colon of Piezo2-Cre mice to specially label colonic afferent neurons. Clozapine N-oxide (CNO, 2 mg/kg body weight intraperitoneal (i.p.) injection) treatment causes colonic hypersensitivity in male mice ( n = 4 biologically independent animals. Data are presented as mean values + /- SEM. Two-tailed paired t test: p = 0.002, t = 7.171, r = 0.7982) but not in female mice ( n = 3 biologically independent animals. Data are presented as mean values + /- SEM. Two-tailed paired t test: p = 0.2426 , t = 1.64, r = 0.9998, no multiple comparisons were made). e Representative images of Piezo2 expression demonstrated by Piezo2-Cre driven mCherry products in DRGs of male and female mice. Scale bar: 50 μm. f Piezo2-immunoreactivity (IR) in DRG of male and female mice. Scale bar: 50 μm. g Summarized data shows the number of Piezo2-expressing neurons in 10 5 µm 2 area of DRG sections of male and female mice. Data are pools from Piezo2;mCherry (male n = 3 biologically independent animals, female n = 2 biologically independent animals) and Piezo2-IR (male n = 2 biologically independent animals, female n = 2 biologically independent animals) totaling n = 5 biologically independent male mice and n = 4 biologically independent female mice. Data are presented as mean values + /- SEM. Two-tailed unpaired t test ( p = 0.0021, t = 4.733, F = 12.83).

    Article Snippet: The retrograde AAV virus (Addgene ID 44361-AAVrg) was injected in the same manner.

    Techniques: Activation Assay, Expressing, Two Tailed Test, Injection